
Cell-Free Fetal DNA Library Prepare Kit(NIPT)
Prenatal screening and prenatal diagnosis are effective means to avoid children with genetic defects.Conventional prenatal screening methods include: combined ultrasound and serology screening in early pregnancy and maternal serological screening in mid-pregnancy.Screening results for high-risk pregnant women are recommended for final confirmation by prenatal diagnosis, and informed choice by pregnant women.The prenatal diagnostic gold standard for fetal chromosomal abnormalities is interventional prenatal diagnostic surgery, which refers to the karyotype of fetal chromosomes by cell biology using the method of villus extraction/amniocentesis/percutaneous umbilical puncture analysis.
This kit employs high-throughput sequencing technology and bioinformatics methods to statistically analyze the proportion of each chromosome's DNA fragments in the plasma, thereby obtaining information on chromosome numbers and enabling rapid and accurate prenatal screening of fetal chromosomal abnormalities.
- SKU:
- Category: High Throughput Solutions
·Product Description
This product is used for qualitative detection of fetal cell-free DNA in the plasma of peripheral blood from pregnant women at gestational weeks 12+0 to 22+6. By combining next-generation sequencing technology with bioinformatics analysis, it determines the risk of the fetus having certain chromosomal disorders.
·Principle
In 1997, Dennis Lo, a professor at the Chinese University of Hong Kong School of Medicine, found that there are fetal free DNA fragments in the maternal peripheral blood called cell-free fetal DNA (cffDNA). The content of these cffDNA in maternal plasma is There is a certain relationship in gestational age and fluctuates within a certain range.When the number of chromosomes in a fetus is abnormal, the cffDNA ratio of the chromosome will exceed the normal range.By sequencing, we can count the proportion of DNA fragments in each chromosome in plasma to obtain information on the number of chromosomes.
This kit is based on the small fragment DNA library construction technology. For plasma free DNA, the end of the small fragment DNA is repaired, an adenine deoxynucleotide is added, and the sequencing universal primer is connected by TA ligation method, and then amplified by PCR technology.
Additional Information
Sample Type
Cell-Free DNA Collection Tube,10mL pregnant woman's whole blood
Performance
① High Library Construction Quality: Includes a complete set of reagents for library preparation, purification, and sequencing; the library construction failure rate of the kit is ≤1%.
② Precision: <5%.
③ Sensitivity: At a DNA concentration of 1.67 ng/mL, the minimum detectable percentage of fetal DNA is 3.5%.
④ High Accuracy: A multi-dimensional calibration strategy is employed to further reduce the false-negative and false-positive rates.
⑤ Strong Anti-interference Capability.
⑥ High Cost-effectiveness:A single test can detect 26/104 types of chromosomal disease
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