Real-time PCR is based on the revolutionary method of PCR, developed in the 1980s, which allows researchers to amplify specific pieces of DNA more than a billionfold. PCR-based strategies have propelled molecular biology forward by enabling researchers to manipulate DNA more easily, thereby facilitating both common procedures, such as cloning, and huge endeavors such as the Human Genome Project. Real-time PCR represents yet another technological leap forward that has opened up new and powerful applications for researchers throughout the world. This is in part because the enormous sensitivity of PCR has been coupled to the precision afforded by “real-time” monitoring of PCR products as they are generated. Real-time PCR instrumentation was first made commercially available in 1996, after which several other companies added new machines to the market.
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How Real-Time PCR Works
Real-time PCR is a variation of traditional, or end-point, PCR technique that is commonly used to quantify DNA or RNA in a sample. Using sequence-specific primers, the number of copies of a particular DNA or RNA sequence can be determined. If a particular sequence (DNA or RNA) is abundant in the sample, amplification is observed in earlier cycles; if the sequence is scarce, amplification is observed in later cycles. Detection and quantification of amplified product is obtained using fluorescent probes or fluorescent DNA-binding dyes and real-time PCR instruments that measure fluorescence while performing the thermal cycling for PCR.
Applications of Real-Time PCR
Real-time PCR is a relatively simple, inexpensive, extremely sensitive and specific tool for determining the expression level of a gene of interest. Real-time PCR is a method for quantitatively determining the copy number of a PCR template, such as DNA or cDNA, and includes two types: probe-based and intercalator-based.
Real-time PCR system Cost
As of January 30, 2019, the list price for a simple PCR machine like the Bio-Rad T100 Thermal Cycler is $4,912 (US sale price is $2,595). The cost of rtPCR systems ranges from $15,000 for some RotorGene models to over $90,000 for the QuantStudio 12k model. However, the price of Real-time PCR system is different upon your reqeust, if you would like to know the latest price of Real-time PCR systmem, feel free to contact us.
Real-time PCR system and Consumables Supplier
As a leader in PCR innovation in China, Geneture have become the gold standard in real-time PCR instrumentation. When you purchase our Applied Biosystems real-time PCR platform, you get exceptional performance, reliability, and first-class support at a great value. You can start your experiments with ease. Feel free to send an inquiry and get cost-effctive real time PCR solutions.