Nucleic Acid Extraction Kit

Animal Genomic DNA Extraction Kit

It is suitable for extracting high-purity genomic DNA from different types of tissues. The genomic DNA (OD260-OD320)/(OD280-OD320) purified by this kit is between 1.7-2.0, which can be used for PCR/RT-PCR, Real -time PCR/Real-time RT-PCR, next-generation sequencing, gene chip and other molecular biology experiments.

This kit uses magnetic beads and buffer system with unique separation function to rapidly, highly sensitively and efficiently separate and purify high-quality genomic DNA from plant samples. The animal cell membranes are destroyed with guanidine salt, surfactant and protease to release genomic DNA in the buffer system, in which specially coated magnetic beads are added. The magnetic beads have a strong affinity to the target DNA in the buffer system, and release the adsorbed DNA when the conditions change, and the washing system removes impurities such as proteins and small molecules in the solution, thus achieving the purpose of rapid separation and purification of genomic DNA without the use of toxic reagents such as chloroform.

Main components: guanidine hydrochloride, superparamagnetic beads, EDTA, tris(hydroxymethyl) aminomethane, and protease K 

Specification

Pre-packed reagent

Lysis buffer AD

Lysis buffer B

Stirrerset

36T/kit (pre-packed)

1T×36 strips

1 vial

1 tube

10 pcs.

20T/kit (pre-packed)

5T/plate×4

1 vial

1 tube

4 pcs.

32T/kit (pre-packed)

8T/plate×4

1 vial

1 tube

4 pcs.

64T/kit (pre-packed)

16T/plate×4

1 vial

2 tubes

8 pcs.






Additional Information

case

Genome extraction effect of Yezo scallop

Starting amount: 20mg of gills, the remaining 30mg, 100 uI elution, 3 ul loading Agarose gel concentration is 1%, 6V/cm, electrophoresis for 20min; M: λ DNAHind II

case

Extraction effect of crucian carp genome
Starting amount: 20mg of gills, the remaining 30mg, 100 uI elution, 3 ul loading Agarose gel concentration is 1%, 6V/cm, electrophoresis for 20min; M: λ DNAHind II


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