PCR, RT-PCR, qPCR

What is PCR?

PCR means polymerase chain reaction, is one of the most powerful technologies in molecular biology. Using PCR, specific sequences within a DNA or complementary DNA (cDNA) template can be copied, or “amplified,” many thousand- to million-fold using sequence-specific oligonucleotides, heat-stable DNA polymerase, and thermal cycling.

PCR is a biochemical process capable of amplifying a single DNA molecule into millions of copies in a short time. Amplification is achieved by a series of three steps: (1) denaturation, (2) annealing, (3) extension, These steps are repeated 25–35 times to exponentially produce exact copies of the target DNA.

What is PCR testing?

PCR refers to polymerase chain reaction. This is a test that detects the genetic material of a specific organism, such as a virus. If you have a virus while being tested, the test will detect the presence of the virus. The test can detect fragments of the virus even if you are no longer infected.

What is a COVID-19 PCR test?

A polymerase chain reaction (PCR) test for COVID-19 is a molecular test that analyzes a sample of your upper respiratory tract for the genetic material (ribonucleic acid or RNA) of SARS-CoV-2, the virus that causes COVID-19. Scientists use PCR technology to amplify small amounts of RNA in a sample into deoxyribonucleic acid (DNA), which replicates until SARS-CoV-2, if present, can be detected. The PCR test has been the gold standard test for diagnosing COVID-19 since it was approved for use in February 2020. It's accurate and reliable.

What is RT-PCR?

A real-time reverse transcription polymerase chain reaction (RT-PCR) assay was developed for the rapid detection of severe acute respiratory syndrome-associated coronavirus (SARS-CoV). Based on multiple primer and probe sets located in different regions of the SARS-CoV genome, this assay can distinguish SARS-CoV from other human and animal coronaviruses with a potential limit of detection of <10 genome copies per reaction. Real-time RT-PCR assays are more sensitive than traditional RT-PCR assays or culture isolation and have proven suitable for the detection of SARS-CoV in clinical specimens. The application of this assay will aid in the diagnosis of SARS-CoV infection.

Geneture's PCR amplification systems feature the latest technological advances, providing greater accuracy and reproducibility in nucleic acid amplification for genomic experiments. Product lines include real-time PCR systems, PCR reagents, plastic products (PCR plates and PCR tubes). If you are looking for something about PCR, just feel free to contact.