Magnetic Beads Details

What are magnetic beads?

Magnetic beads are a special class of nano-micron materials. Their size is usually between a few tenths of a micron and a few microns, which is only one-tenth to several tenths of the diameter of a hair and cannot be observed by the naked eye. of a single magnetic bead. They are superparamagnetic and can move quickly to one side in a magnetic field, clump together, and quickly return to a dispersed state when the magnetic field is removed.

Geneture Magnetic beads product features:

Reagent bottle packaging or packaging according to customer requirements.

High throughput automation;

Simple operation, high efficiency;

Safe and non-toxic;

Good purity, high yield;

High sensitivity.

Introduction of nucleic acid extraction method by magnetic bead method:

The surface of magnetic beads used for nucleic acid extraction has specific properties, which can adsorb viral nucleic acid on the surface under certain conditions, and release viral nucleic acid from the surface under other conditions for subsequent detection steps. Nucleic acid binding to magnetic beads mainly relies on electrostatic interaction, hydrophobic interaction and hydrogen bonding. Under the action of lysate, the DNA/RNA in cells or tissues are released. At this time, the surface-modified superparamagnetic silicon oxide nano-magnetic beads "specifically bind" to the nucleic acid to form a "nucleic acid-magnetic bead complex". Then under the action of an external magnetic field, the complex is separated. Finally, after washing off the non-specifically adsorbed magazines, desalting, and purifying with the eluent, the nucleic acid substance to be extracted is obtained.

Advantages of magnetic bead nucleic acid extraction:

With the popularization of genetic testing, personalized medicine, prenatal diagnosis, etc., high-throughput and automation are pursued in all fields of the biological industry, the limitations of traditional DNA extraction methods are becoming more and more obvious, while the magnetic bead method for DNA extraction The advantages are becoming more and more obvious: it can realize automation and large-scale operation; the operation is simple and the time is short; the toxic reagents such as benzene and chloroform in the traditional method are not used, and the safety and non-toxicity are completely in line with the modern environmental protection concept; the specific combination with nucleic acid makes The extracted nucleic acid has high purity and high concentration.

Magnetic bead separation principle:

The surface of nano-scale magnetic beads is modified with specific reactive functional groups, which can adsorb nucleic acids.

By using different lysing solutions, binding solutions and washing solutions, it can specifically bind to the target nucleic acid under specific conditions, and by using magnetic beads with their own magnetism, under the action of an external magnetic field, it is easy to achieve directional movement and Enrichment, so as to achieve the purpose of separating nucleic acids and impurities, and then achieve the separation and purification of target substances to obtain purified nucleic acids.

The magnetic bead method does not require centrifugation, is simple to operate, and is suitable for automatic extraction. The entire extraction process takes a very short time. At present, most nucleic acid extractors on the market use magnetic bead separation to achieve automation.

Application of magnetic bead nucleic acid extraction:

Magnetic bead application: antibody purification, molecular separation, cell detection, microbial separation, chemiluminescence, etc.

Using nanotechnology to improve and modify the surface of superparamagnetic nanoparticles, superparamagnetic silicon oxide nano-magnetic beads are prepared. The magnetic beads can specifically recognize and efficiently bind to nucleic acid molecules on a microscopic interface. Using the superparamagnetic properties of silica-coated nanomagnetic microspheres, under the action of Chaotropic salts (guanidine hydrochloride, guanidine isothiocyanate, etc.) and an external magnetic field, samples from blood, animal tissues, food, pathogenic microorganisms, etc. The isolated DNA and RNA can be used in clinical disease diagnosis, blood transfusion safety, forensic identification, environmental microbial testing, food safety testing, molecular biology research and other fields.

Supplier of Magnetic beads:

GENETURE is a group company focus on the field of clinical diagnosis and life sciences,provide one stop solution of Nucleic Acid Extraction and Analysis,including solution of COVID-19. Main products including: Nucleic acid extraction or purification kit,Automatic nucleic acid extractor, PCR system, PCR kit, Magnetic beads, and lab consumables of 96 well deep plate,Magnetic rod comb,PCR tube,PCR plate,Pipette tips,centrifuge tubes.etc.

If you have any requirements or questions about Magnetic beads, please feel free to contact us.