Bacterial Genomic DNA Extraction Kit
The bacterial genomic DNA nucleic acid extraction kit uses magnetic beads to adsorb DNA to achieve the purpose of rapid purification of bacterial genomic DNA. It is suitable for extracting high-purity genomic DNA from different bacterial samples. The genomic DNA (OD260-OD320)/(OD280-OD320) purified by this kit is between 1.7 and 2.0. It can be used for PCR/RT-PCR, Real -time PCR/Real-time RT-PCR, next-generation sequencing, gene chip and other molecular biology experiments.
- SKU:
- Category: Nucleic Acid Extraction Kit
This kit uses magnetic beads with a unique separation function and a buffer system to rapidly, highly sensitively and efficiently isolate and purify high-quality genomic DNA from plant samples. The plant cell membranes are destroyed with guanidine salt, surfactant and protease to release genomic DNA in the buffer system, in which specially coated magnetic beads are added. The magnetic beads have a strong affinity for the target DNA in the buffer system, and release the adsorbed DNA when the conditions change, and the washing system removes impurities such as proteins and small molecules in the solution, thus achieving the purpose of rapid separation and purification of genomic DNA without the use of toxic reagents such as chloroform.
Bacterial Genomic DNA Extraction Kit |
Lysis AX |
Lysis Solution B |
Dissolving solution A |
Magnetic beads A |
Washing solution A |
Washing liquid S |
Eluent A |
Additional Information
Measured values of extraction products of different bacterial species.
Strain | Numbering | concentration(μg/ml) | OD260/280 | OD260/230 | A340 |
Bacillus cereus (OD600=0.410) | 1 | 76.9 | 2.01 | 2.38 | 0.000 |
2 | 82.0 | 2.01 | 2.26 | 0.009 | |
3 | 85.5 | 1.95 | 2.10 | 0.000 | |
4 | 96.5 | 1.91 | 2.12 | 0.019 | |
Staphylococcus aureus(OD600=0.919) | 1 | 263.0 | 1.89 | 2.09 | 0.037 |
2 | 323.5 | 2.02 | 2.44 | 0.015 | |
3 | 270.2 | 1.99 | 2.20 | 0.016 | |
4 | 296.9 | 1.96 | 1.31 | 0.008 | |
Bacillus subtilis(OD600=0.286) | 1 | 265.1 | 2.13 | 2.52 | 0.000 |
2 | 241.1 | 2.09 | 2.43 | 0.004 | |
3 | 302.9 | 2.03 | 2.30 | 0.023 | |
4 | 306.3 | 1.94 | 2.12 | 0.045 | |
Escherichia coli (OD600=0.786) | 1 | 113.8 | 1.82 | 1.92 | 0.021 |
2 | 128.9 | 1.92 | 2.00 | 0.013 | |
4 | 119.9 | 1.93 | 2.05 | 0.013 | |
5 | 199.2 | 1.97 | 2.10 | 0.015 |
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