5 types DNA extraction methods from human whole blood samples
By now, there are 5 main types of DNA extraction method:
1 Solution-based DNA extraction methods
1.1 Solution-based DNA extraction methods using organic solvents
1.2 Solution-based DNA extraction methods using salting out
2 Solid-phase DNA extraction methods
2.1 DNA extraction methods using silica and silica matrices
2.2 DNA extraction using anion exchange resins
2.3 DNA extraction methods using magnetic beads
Nucleic acid extraction techniques using magnetic separation have been emerging since the early 1990s. Magnetic particles are made of one or several magnetic cores, such as magnetite (Fe3O4) or maghemite (gamma Fe2O3). coated with a matrix of polymers, silica, or hydroxyapatite with terminal functionalized groups.
The surface of nano-scale magnetic beads is modified with specific active functional groups that can adsorb nucleic acids. By using different lysing solutions, binding solutions, and washing solutions, they can specifically bind to the target nucleic acid under specific conditions, while using magnetic beads with its own magnetism, it can easily achieve directional movement and enrichment under the action of an external magnetic field, so as to achieve the purpose of separating nucleic acid and impurities, and then achieve the separation and purification of target substances to obtain purified nucleic acid.
The magnetic bead method does not require centrifugation, is simple to operate, and is suitable for automated extraction. The entire extraction process takes a short time. At present, most nucleic acid extractors on the market use magnetic bead separation to achieve automation.
Ascend Whole Blood Genomic NDA Nucleic Acid Extraction Kit 50T/64T uses magnetic beads to adsorb DNA to achieve the purpose of rapid purification of whole blood genomic DNA. It is suitable for extracting high-purity genomic DNA from 150-200 µl anticoagulated whole blood samples. This buffer system can remove protein, pigment, lipid and other inhibitory impurities to the maximum extent. The extracted genomic DNA fragment is large, the yield is high, the purity is good, and it is stable and reliable.
Whole blood genome NDA nucleic acid extraction kit 50T avoids the use of organic solvents such as phenol and chloroform, and the recovered DNA can be applied to various routine operations, such as enzyme digestion, PCR, library construction, Southern hybridization and other experiments.
The Whole Blood Genomic NDA Nucleic Acid Extraction Kit is matched with a unique buffer, which can better lyse red blood cells to release DNA and increase the yield, but also has less damage to genomic DNA, which can protect the integrity of DNA.
For more information, questions, or support about nucleic acid extraction (Magnetic Bead Method), please feel free to contact