Advantages of Magnetic Bead Method Extraction of DNA
Nucleic acids are masters in the biological world and are a very important class of biological macromolecules. They play an important role in all life activities such as reproduction, development, maintenance, aging and death of organisms. At present, the nucleic acid has become a biochemical, genetics, and other popular research topic related to life sciences, whose coverage and speed of progress are unmatched by other disciplines. Today, I will share with you DNA extraction by magnetic bead method, hoping to help you.
DNA extraction principles:
Ensure the integrity of the DNA primary structure;
There should be no organic solvent and high concentration of metal ions that inhibit the enzyme in the extracted DNA sample;
Contamination of other biological macromolecules such as proteins, polysaccharides, and lipid molecules should be minimized;
Contamination by other nucleic acid molecules (RNA) is excluded.
The experimental steps of DNA extraction by magnetic bead method
Magnetic bead nucleic acid extraction can generally be divided into four steps:
Lysis - Binding - Washing - Elution
1. Cracking
Nucleic acids must be released from cells or other biological substances, and cell lysis can be achieved by mechanical, chemical, enzymatic and other methods. Among them, the enzyme action is mainly through the addition of lysozyme or protease (proteinase K, plant protease or pronase) to break cells and release nucleic acids. Proteases can also degrade proteins bound to nucleic acids and facilitate the separation of nucleic acids.
2. Combine
The surface of the magnetic bead is negatively charged, and the magnetic bead buffer is a high-salt environment with positively charged salt ions. The sample is affected by the buffer, and the phosphate group is negatively charged.
3. Washing
The highly charged phosphate backbone of nucleic acids makes them more hydrophilic than other biological macromolecules such as proteins, polysaccharides, and fats. According to their differences in physicochemical properties, nucleic acids can be separated by methods such as selective precipitation, chromatography, and density gradient centrifugation. ,purification.
Precipitate the DNA with absolute ethanol, which is the most commonly used method for precipitating DNA in experiments. The advantage of ethanol is that it can be miscible with water in any ratio. Ethanol does not have any chemical reaction with nucleic acid and is very safe for DNA, so it is a rational precipitant. When ethanol is added, ethanol will take away the water molecules around the DNA, dehydrating the DNA and making it easier to polymerize.
4. Elution
Adding water or EB destroys the high-salt environment and forms a low-salt environment, which causes the DNA to fall off the magnetic beads.
The working principle of DNA extraction by magnetic bead method
The magnetic bead method is to lyse the cell tissue sample by lysing solution. The nucleic acid molecules released from the sample are specifically adsorbed to the surface of the magnetic particles, and impurities such as proteins are not adsorbed and remain in the solution. The nucleic acid-carrying magnetic beads are moved to different reagent tanks, and the pure nucleic acid is finally obtained by repeated rapid stirring and mixing of the liquid, cell lysis, nucleic acid adsorption, washing and elution.
The principle of DNA extraction by magnetic beads: the action of charged salt ions (such as Na+), the negatively charged phosphate group forms an ion bridge with the carboxyl group through the dissociated salt ion (such as Na+) so that DNA is specifically adsorbed to the carboxyl magnetic beads surface. When PEG and salts are removed, adding water-based molecules will quickly and fully hydrate the DNA, releasing the ionic interaction between the three, so that the DNA adsorbed to the magnetic beads is purified.
Advantages of Magnetic Bead Extraction of DNA
The magnetic bead method is a perfect combination of nanotechnology and biotechnology, and has incomparable advantages over other DNA extraction methods, mainly reflected in the:
1. It can realize automatic and high-throughput operations. With the 96-well nucleic acid automatic extraction instrument, 96 samples can be processed simultaneously in the extraction time of one sample in the past, and the efficiency is directly increased by 96 times, which meets the current biological requirements. The requirement of high-throughput operation enables rapid screening of causes in large-scale outbreaks, an advantage that other methods are difficult to catch up with.
2. The operation is simple and the time is short. The entire extraction process can be completed in a short time with only four steps of cracking, binding, washing, and elution.
3. It is safe and non-toxic, and does not use toxic reagents such as benzene and chloroform in traditional methods, which causes less damage to the experimental operators and protects the physical health of the experimental personnel.
4. The specific combination of magnetic beads and nucleic acid makes the extracted nucleic acid high in purity and concentration.
5. High sensitivity, suitable for trace DNA extraction such as forensic samples.
The magnetic bead method nucleic acid extraction kit is a high-tech product combining biological science and nanomaterial science, and a major breakthrough in nucleic acid extraction and purification technology. Genetic testing will also become a new symbol of the development of the biological industry. The emergence of high-throughput, automated nucleic acid extraction methods will reduce the labor cost of genetic testing and make large-scale testing a reality.
Professional manufacturer of Nucleic Acid (DNA & RNA) Extraction and Analysis products Magnetic Beads supplier
GENETURE is a group company,we own two factories: Ascend and Dianrun,to provide one stop solution of Nucleic Acid Extraction and Analysis,including solution for COVID-19. Geneture provides high quality and professional Nucleic Acid Extraction Reagents, Lab consumables, Real-time PCR consumables and test machines.
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