What is the magnetic bead nucleic acid extraction?
1. What is the magnetic bead method?
The magnetic bead method, also known as a pipetting method, is to extract nucleic acid by fixing magnetic beads and transferring liquid. Generally, the manipulator is controlled by the operating system to achieve the transfer. The extraction process is as follows:
2. What is the magnetic bead process?
Lysis: The lysate is added to the sample, heated by mechanical movement, cells are lysed, the nucleic acid is released, and the reaction solution is mixed and fully reacted.
Adsorption: Add magnetic beads to the sample solution and mix thoroughly. Under the condition of high salt and low pH, magnetic beads have a strong affinity for nucleic acid and can adsorb nucleic acid. Under the action of the external magnetic field, the magnetic beads are separated from the solution, and the liquid is removed by the tip, discarded into the waste liquid tank, and the tip is discarded.
Cleaning: remove the excluded magnetic field, replace the new tip, add the washing buffer solution, thoroughly mix to remove impurities, and remove the liquid under the external magnetic field.
Elution: Remove the external magnetic field, replace the probe with a new one, add elution buffer, and mix thoroughly. The bound nucleic acid was separated from the magnetic beads to obtain the purified nucleic acid
3. Preventive measures
The installation environment of the instrument: normal pressure, temperature 20-35℃, typical operating temperature 25℃, relative humidity 10%-80%, unobstructed airflow 35℃ and below.
Avoid placing the instrument near heat sources, such as electric heaters; At the same time, to prevent electrical components from short circuits, avoid splashing water or other liquid into it.
The inlet and outlet are located at the back of the instrument, and avoid dust or fiber gathering in the inlet to keep the air duct unobstructed.
The nucleic acid extractor should be at least 10cm away from other vertical surfaces.
Instrument grounding: To avoid electric shock, the input power cable of the instrument must be grounded.
Keep away from living circuits: The operator must not disassemble the instrument without authorization. Replacement parts or internal adjustments must be made by qualified professional maintenance personnel. It is forbidden to replace parts with electricity
4. The characteristics of the Genuture magnetic bead method nucleic acid extractor
Automated, high-throughput operation
The operation is simple and quick.
Safe and environmentally friendly.
High purity, high yield.
No pollution, stable effect.
Low cost, wide application range.
Can simultaneously process different types of samples.
Genetic Medicine provides one-stop nucleic acid extraction solutions. Please contact us for more information.
Professional manufacturer of Nucleic Acid (DNA & RNA) Extraction and Analysis products supplier
GENETURE is a group company,we own two factories: Ascend and Dianrun,to provide one stop solution of Nucleic Acid Extraction and Analysis,including solution for COVID-19. Geneture provides high quality and professional Nucleic Acid Extraction Reagents, Lab consumables, Real-time PCR consumables and test machines.
GENETURE main products including: Nucleic acid extraction or purification kit,Automatic nucleic acid extractor, PCR system, PCR kit, Magnetic beads, and lab consumables of 96 well deep plate,Magnetic rod comb,PCR tube,PCR plate,Pipette tips,centrifuge tubes.
Geneture's PCR amplification systems feature the latest technological advances, providing greater accuracy and reproducibility in nucleic acid amplification for genomic experiments. Product lines include real-time PCR systems, PCR reagents, and plastic products (PCR plates and PCR tubes). If you have any questions about PCR, feel free to contact us.
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