Nucleic Acid Extraction Kit

Nucleic Acid Extraction Kit(Magnetic Beads)-cfDNA

This kit was used to isolate and purify high-quality cell-free DNA fragments from 1-4 ml plasma. This process does not contain toxic reagents such as phenol and chloroform, and uses magnetic beads and buffer system with unique separation effect, which is fast, highly sensitive and effective to extract the target cfDNA from plasma samples. The kit is based on specially coated magnetic beads, which have a strong affinity for the target nucleic acid under the buffer system. When the conditions change, the magnetic beads release the adsorbed nucleic acid, and the washing system can remove the impurities such as proteins and small molecules in the solution, which can achieve the purpose of rapid separation and purification of nucleic acid. Finally, the target nucleic acid is eluted under the action of the eluent. With the Geneture Amy 16L large volume automatic nucleic acid extractor, the whole process can be operated, and the sample can be added directly. The extracted genomic DNA has high quality, good purity, large fragment and good stability, which can be directly used for NGS, PCR, enzyme digestion and hybridization experiments.


Model20T

Product Features:

1. Simple operation: no need for external lysis, one-step extraction;

2. Safe and non-toxic: the reagent does not contain toxic substances such as phenol and chloroform.

3. Automatic extraction: with a 16-channel nucleic acid extractor, 16 samples were extracted at one time within 1 hour.

 

Storage Condition:

Lysis Buffer B was transported at room temperature and stored at 2-8 ℃ for long-term storage. The remaining components are transported at room temperature and can be stably stored at room temperature (15-25) °C for 18 months.

Additional Information

A 2mL plasma sample was extracted, the concentration was determined using Qubit, and the fragment length was determined.

Sample ID

Geneture Product

Competitive Product

Elution VolumeμL

Conc. (ng / μl)

Conc. (ng / μl)

Sample 1

133.6

118.75

30

Sample 2

125.9

103.8

Sample 3

102.2

95.6



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