How to choose the nucleic acid extraction system?
what is the nucleic acid extraction system?The nucleic acid extraction system is an instrument that automatically completes the nucleic acid extraction work of samples by applying matching nucleic acid extraction reagents. It is widely used in various fields such as the Center for Disease Control, clinical disease diagnosis, blood transfusion safety, forensic identification, environmental microbiological testing, food safety testing, animal husbandry and molecular biology research.
Nucleic acid extraction system classification
1. Divided according to the size of the instrument model
1.1 Automatic liquid workstation
The automatic liquid workstation is a very powerful device, which automatically completes liquid dispensing and aspiration, and can even realize full automation of specimen extraction, amplification, and detection by integrating functions such as amplification and detection. Nucleic acid extraction is only one application of its function, and it is not suitable for routine laboratory extraction of nucleic acid. It is generally applied to the experimental needs of a single type of specimen and a very large amount of specimens (at least 96, generally several hundred) at a time. The platform establishment and operation of automatic workstations require relatively large funds.
1.2 Small-scale automatic nucleic acid extraction system The small-scale automatic instrument achieves the purpose of automatic nucleic acid extraction through the particularity of the operating structure, and can be used in any laboratory.
2. Differ according to the extraction principle
2.1 Instruments using spin column method
The centrifugal column method nucleic acid extraction system mainly uses a combination of a centrifuge and an automatic pipetting device. The throughput is generally 1-12 samples. The operation time is about the same as manual extraction. It does not improve the actual work efficiency and is expensive. Different models The consumables of the instrument are not universal, and are only suitable for large-scale laboratories with sufficient funds.
2.2 Instruments using magnetic bead method
Using magnetic beads as a carrier, using the principle of magnetic beads adsorbing nucleic acids under high salt and low pH values, and separating them from nucleic acids under low salt and high pH values, the entire nucleic acid extraction and purification process is realized by moving the magnetic beads or transferring the liquid. Due to its unique principle, it can be designed into a variety of fluxes. It can be extracted from a single tube or 8-96 samples, and its operation is simple and fast. It only takes 10-30min to extract 96 samples, which greatly improves The efficiency of the experiment and the low cost allow it to be used in different laboratories. It is currently the mainstream instrument on the market.
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