What is cell culture technology?
What is cell culture technology?Cell culture technology refers to the growth of cells under in vitro conditions, during which the cells no longer form tissue (animals).
A culture is a single cell or group of cells. Cells must live in an artificial environment when they are cultured. Due to changes in the environment, the movement of cells or the influence of some other factors, the culture time is prolonged, and the passage leads to a single type of cells.
Advantages and disadvantages of cell culture techniques in research applications.
Advantage：
1. Directly observe the morphological structure and life activities of living cells. It is used for research in various disciplines such as cytology, genetics, immunology, experimental medicine and oncology.
2. Direct observation of cell changes can facilitate photography.
3. Study cell types such as low to high to human, embryo to adult, normal tissue to tumor.
4. It is convenient to use various techniques: phase contrast, fluorescence, electron microscopy, histochemistry, isotope labeling and other methods to observe and study cell conditions.
5. It is the research object of molecular biology and genetic engineering, and it is also its main component.
6. It is easy to apply physical and chemical biological experiments.
7. It is easy to provide a large number of experimental objects with similar biological properties, and the cost is less and more economical.
8. Become a source of materials for the production of monoclonal antibodies and genetic engineering of biological products.
Disadvantages：
Tissues and cells live independently in an artificial culture environment after ex vivo, and although they simulate the in vivo environment, there are still great differences. Therefore, when using cultured cells for experiments, it should not be considered that the cells in the body are exactly the same.
What are the cell types?
Cell types are classified into attached type and suspension type according to the characteristics of whether they are attached to the support or not.
Types of adherent cells
1. Fibroblasts are derived from the mesoderm
Features: Similar in shape to fibroblasts in vivo, the cell body is fusiform or irregular triangular, with a round nucleus in the center, and the cytoplasm protrudes outward with 2 to 3 protrusions of different lengths. Cells grow radially, swirl or flame.
Origin: Cells are derived from mesoderm mesenchymal tissue.
In addition to true fibroblasts, cardiac muscle, smooth muscle, osteoblasts, vascular endothelial cells.
Note: It is customary for cells to become fibroblast-type cells in culture, as opposed to cells in vivo.
2. Epithelial cells are derived from ectoderm.
Features: Flat irregular polygonal shape, round nuclei, closely connected cells, and the entire epithelial membrane moves when the number of cells proliferates increases. Marginal cells rarely break away from the cell group and act alone. The phenomenon of "pulling the net" is related to the origin of the ectoderm tissue.
Tissue: skin epidermis and its derivatives (sweat glands, sebaceous glands) digestive tract is divided into epithelium, liver, pancreas, alveolar epithelium.
3. Wandering cells
Features: Scattered growth on the support, not connected into sheets, cytoplasmic protruding pseudopodia or protrusions, active wandering or deformation movement, fast and irregular, high density, connected into sheets and polygonal, not easy to connect with other types of cells the difference.
4. Polymorphic cells
Features: Irregular morphology, such as nerve cells.
Suspended
Features: Do not attach to the support to grow, the cell body is round, the growth space in the culture medium is large, it can grow for a long time, and the reproduction is vigorous, which is convenient for cell metabolism research. S180 sarcoma, leukocytes in blood, K562, HL-60.
The purpose of typing:
It is convenient to describe the morphological changes of cells during culture. When the culture conditions are good, the cells are relatively stable, which can reflect the difference between origin and normal abnormality, and can be used as an indicator to determine the biological characteristics of cells.
Emphasis: The general pattern is not a reliable indicator to be affected by all aspects.
Laboratory consumables supplier:
Geneture medical specialized in the field of nucleic acid extraction system, PCR detection system and laboratory consumables, main products involved in：
1.Auto nucleic Acid Extractor （32T, 96T）
2.PCR fluorescence quantitative analyzer (16T, 96T)
3.Nucleic Acid Extraction Kit (Magnetic beads method)
4.Lab consumables: deep well plate, mag-rod sleeve, pipette tips, VTM, swabs, PCR tubes, PCR plate, centrifuge tubes, cryogenic Vials, saliva collection, cell culture dish/flask/plate/tube and so on.
Please don’t hesitate to contact with us to get a quotation.